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This hypothesis based observations that cleavage the antigen in vitro chemical means yields poisonous split products that combination specific serum with its corresponding antigen yields toxic substances, identical in their physiological action with those produced from the antigen chemically and, lastly, that the actual cleavage antigen the ferments specific senun can demonstrated in vitro. There exists, however, another view the origin so-called anaphylatoxin. This view represented the work Nolf, Doerr, Ritz and Sachs, Bordet, and others known as physical theory.
In the main this theory suggests that the source anaphylatoxin where can i find research papers not the antigen, but the protein the serum Itself.
As mentioned online dissertation writing above, in his original experiments, Friedbeiger has succeeded in producing the toxic substances in vitro a process similar that which was supposed responsible for the anaphylactic shock in vivo. Namely, allowed antigen combine with its specific antibody and added the complement this mixture. The poisons obtained in this manner in vitro, when injected into normal animals, were able produce typical anaphylactic symptoms.
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In his later experiments, however, Friedberger succeeded in obtaining similar poisons from bacteria and other proteins their incubation with normal guinea-pig complement, without the concurrence specific antibody.
He still believed, however, that in these experiments also the poison was derived from the protein the substratum.
Even when Bordet, Nathan, Mutermilch, and others have been able obtain similar poisons the incubation normal guinea-pig senun with agar, starch, or kaolin, and have decided, therefore, that the poison must originate from the serum, Friedbeiger still objected these conclusions the basb that these substances may contain a small amount protein impurities, which really furnish the substratum for the formation anaphylatoxin. In studying this controversy one finds two really independent questions involved the term anaphylatoxin was applied Friedberger designate the poison which was supposed identical with the one produced in vivo during the anaphylactic shock.
This poison had two essential characteristics, i possible assimie its identity with the substance causing anaphylaxis First, the method its production from pombination antigen with antibody and complement, and, second, its physiological action upon normal animals, which identical with anaphylactic shock.
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In discussing the question the origin anaphylatoxin different authors apparently used as criterion in their terminology only the second characteristic the original anaphylatoxin namely, its physiological effect upon normal animals. Thus many authors have called anaphylatoxin the chemical poisons obtained Vaughan from bacteria.
Nathan, Bordet, and Mutermilch called anaphylatoxins, substances obtained them from the serum adsorption with inert substances, etc. Attempts these authors explain the nature anaphylatoxin seem generally inadequate because, even though, as experiments have also confirmed, digestion senmi with kaolin, for instance, one can produce from senun a substance which similar in its physiological action, the one hand, the original anaphylatoxin Friedberger, and, the other hand, the chemical poison Vaughan, its identity with one or the other not proved this similarity alone. The identity between all these substances has proven before the experiments above could taken into consideration in discussing the probable nature anaphylatoxin. This attempted in a series experiments in which studied the actual mechanism the production poison in each instance. These experiments have shown that in the poisons arising during the incubation the serum with kaolin or starch, as well as those arising when scholarship essay writing help specific serum digested dissertation research consultant with its antigen, the rum the source poisonous products. Moreover, was found that the products such autodigestion admission essay service senun are toxic write my persuasive paper only homologous animals. This would indicate that during the anaphylaxis in vivo the toxic split products originate only from the digestion serum or tissues the animal and not from heterologous protein the antigen though the latter may also attacked the ferment at the same Since doctoral dissertation help established that in the experiments best website to buy a research paper Bordet, as well as in those Friedberger, the formation toxic split products identical namely, that due the digestion the normal senmi ferments autogenous protein the similarity the biologic properties the respective end products such digestion may speak for their identity. On the other hand, the fact that in Vaughan's experiments the poison arises from heterologous protein shows that this poison not identical with anaphylatoxin, in spite the similarity its biologic action upon animals. These findings, taken in connection with the results obtained in the study the mechimism specific parenteral digestion, referred above, suggest that the nature anaphylatoxin as follows Fresh serum owl purdue paraphrasing contains normal proteolytic ferments whose digestive action in vivo as well writing help for kids as in vitro inhibited the simultaneous presence some antitryptic elements. This antitrypsin can removed from the serum in vitro two independent processes buy papers online one, nonspecific, a simple mechanical adsorption means excess some organic as well as some inoiganic substances the other specific, an inactivation the antitryptic properties the serum, taking place as a result the physico-chemical changes in the serum, induced the specific interaction between the antigen and the antibody the immune serum. The removal the inhibiting antitryptic action the serum either method followed the restitution the activity the normal proteolytic enzyme, which may attack both the protein the antigen as well as the protein the serum itself.
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